Mason Archival Repository Service

Exosomes from Uninfected Cells Cause Transcriptional Activation of Latent HIV-1 Virus in Infected Immune Cells

Show simple item record

dc.contributor.advisor Kashanchi, Fatah
dc.contributor.author Barclay, Robert A
dc.creator Barclay, Robert A
dc.date 2016-07-29
dc.date.accessioned 2017-10-03T17:35:59Z
dc.date.available 2017-10-03T17:35:59Z
dc.identifier doi:10.13021/G8T685
dc.identifier.uri http://hdl.handle.net/1920/10765
dc.description.abstract HIV-1 infection causes AIDS, a significant problem in today’s world. It can be combatted through the use of combination antiretroviral therapy, which can lower viral load to undetectable levels. However, because HIV-1 is able to integrate its genome with the host cell’s, achieving latency, there is no effective way to target the viral reservoir. In previous studies, it has been shown that there is a link between HIV-1 and exosomes. Specifically, exosomes were shown to transport viral proteins and RNA from infected cells to neighboring uninfected cells. These viral products could then suppress the PKR pathway, leading to increased pathogenesis. In this study, exosomes from uninfected cells were observed to increase short and long-length viral transcripts within wild-type HIV-infected cells. This effect was again observed when infected cells were under cART. An investigation into a possible mechanism for this phenomenon revealed that the exosomes potentially cause an increase in RNA Polymerase II activity within the infected cells. Collectively, these results imply that exosomes from uninfected cells cause an activation of HIV-1 from latency in infected cells. en_US
dc.language.iso en en_US
dc.subject HIV en_US
dc.subject exosomes en_US
dc.subject latency en_US
dc.subject transcription en_US
dc.title Exosomes from Uninfected Cells Cause Transcriptional Activation of Latent HIV-1 Virus in Infected Immune Cells en_US
dc.type Thesis en_US
thesis.degree.name Master of Science in Biology en_US
thesis.degree.level Master's en_US
thesis.degree.discipline Biology en_US
thesis.degree.grantor George Mason University en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search MARS


Browse

My Account

Statistics