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Total Anthocyanin Levels in Commercially-Available Pigmented Grain Products

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dc.contributor.advisor Slavin, Margaret Hauver, Alexandra
dc.creator Hauver, Alexandra 2016-06-23 2017-10-03T17:36:19Z 2017-10-03T17:36:19Z
dc.identifier doi:10.13021/G8DX11
dc.description.abstract This thesis reports an observational research project, which analyzed the anthocyanin content of products with black soybeans or blue corn as an ingredient. The objectives of the study were: 1) gather a minimum of 8 commercially available food products with black soybean or blue corn as predominant ingredients, and produce triplicate anthocyanin-rich extractions of each, 2) assess anthocyanin content of each extraction by two methods: HPLC to measure the common anthocyanins as determined by a literature search, and the pH differential colorimetric assay for assessing total anthocyanin content, and 3) compare anthocyanin content of processed pigmented grain products to known values for fruit, and amounts demonstrated to be necessary for achieving health benefits. Total anthocyanin content determined by colorimetric assay ranged from 106.1 mg/kg to 261.5 mg/kg full-fat chip, while yellow corn chips contained a mean of 3.8 mg/kg full-fat chip. All six brands of blue corn tortilla chips had a significantly (p<0.05) higher anthocyanin content than yellow corn chips. HPLC analysis of the individual anthocyanins in blue corn tortilla chips showed the presence of 5 out of 6 of the common anthocyanins. Cyanidin-3-glucoside was the most prominent anthocyanin in blue corn tortilla chips ranging from 13.3 mg/kg full-fat chip to 29.5 mg/kg full-fat chip. Pelargonidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, and petunidin-3-glucoside were all present in blue corn chips listed in order of highest quantity. Malvidin-3-glucoside was not found to be present in any of the blue corn chip samples, while no individual anthocyanins were detectable in the yellow corn tortilla chips. The anthocyanins in black soybean milks were unable to be extracted. In a 100 g portion, the amount of anthocyanins found in blue corn tortilla chips is comparable to the amount found in plums. en_US
dc.subject anthocyanins en_US
dc.subject blue corn en_US
dc.subject HPLC en_US
dc.subject food processing en_US
dc.title Total Anthocyanin Levels in Commercially-Available Pigmented Grain Products en_US
dc.type Thesis en_US Master of Science in Nutrition en_US Master's en_US Nutrition en_US George Mason University en_US

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