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An expanded nuclear phylogenomic PCR toolkit for Sapindales

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dc.contributor.author Collins, Elizabeth S.
dc.contributor.author Gostel, Morgan R.
dc.contributor.author Weeks, Andrea
dc.date.accessioned 2018-08-02T18:14:57Z
dc.date.available 2018-08-02T18:14:57Z
dc.date.issued 2016-12-07
dc.identifier.citation Collins, Elizabeth S., Morgan R. Gostel, and Andrea Weeks. “An Expanded Nuclear Phylogenomic PCR Toolkit for Sapindales.” Applications in Plant Sciences 4, no. 12 (December 1, 2016): 1600078. https://doi.org/10.3732/apps.1600078. en_US
dc.identifier.isbn 10.3732/apps.1600078
dc.identifier.uri https://hdl.handle.net/1920/11070
dc.description.abstract Premise of the study: We tested PCR amplification of 91 low‐copy nuclear gene loci in taxa from Sapindales using primers developed for Bursera simaruba (Burseraceae). Methods and Results: Cross‐amplification of these markers among 10 taxa tested was related to their phylogenetic distance from B. simaruba. On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16–90). Arabidopsis thaliana (Brassicales), by contrast, yielded product for two. Single representatives of Anacardiaceae and Rutacaeae yielded 34 and 26 products, respectively. Twenty‐six primer pairs worked for all Burseraceae species tested if highly divergent Aucoumea klaineana is excluded, and eight of these amplified product in every Sapindalean taxon. Conclusions: Our study demonstrates that customized primers for Bursera can amplify product in a range of Sapindalean taxa. This collection of primer pairs, therefore, is a valuable addition to the toolkit for nuclear phylogenomic analyses of Sapindales and warrants further investigation.
dc.description.sponsorship National Science Foundation. Grant Number: 1403150 Provost/COS/ESP Institutional Graduate Fellowship Research National Science Foundation en_US
dc.language.iso en_US en_US
dc.publisher Wiley en_US
dc.rights Attribution-NonCommercial 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0/us/ *
dc.subject Anacardiaceae en_US
dc.subject Burseraceae en_US
dc.subject low‐copy nuclear genes en_US
dc.subject microfluidic PCR en_US
dc.subject Rutaceae en_US
dc.title An expanded nuclear phylogenomic PCR toolkit for Sapindales en_US
dc.type Article en_US


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