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Chemokine-Releasing Microparticles Improve Bacterial Clearance and Survival of Anthrax Spore-Challenged Mice

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dc.contributor.author Popova, Taissia G.
dc.contributor.author Teunis, Allison
dc.contributor.author Espina, Virginia
dc.contributor.author Liotta, Lance
dc.contributor.author Popov, Serguei G.
dc.date.accessioned 2018-08-07T16:11:19Z
dc.date.available 2018-08-07T16:11:19Z
dc.date.issued 2016-09-15
dc.identifier.citation Popova, Taissia G., Allison Teunis, Virginia Espina, Lance A. Liotta, and Serguei G. Popov. “Chemokine-Releasing Microparticles Improve Bacterial Clearance and Survival of Anthrax Spore-Challenged Mice.” PLOS ONE 11, no. 9 (September 15, 2016): e0163163. https://doi.org/10.1371/journal.pone.0163163. en_US
dc.identifier.uri http://hdl.handle.net/1920/11075
dc.description.abstract In this study the hydrogel microparticles (MPs) were used to enhance migration of neutrophils in order to improve outcome of anthrax infection in a mouse model. Two MP formulations were tested. In the first one the polyacrylamide gel MPs were chemically coupled with Cibacron Blue (CB) affinity bait. In the second one the bait molecules within the MPs were additionally loaded with neutrophil-attracting chemokines (CKs), human CXCL8 and mouse CCL3. A non-covalent interaction of the bait with the CKs provided their gradual release after administration of the MPs to the host. Mice were challenged into footpads with Bacillus anthracis Sterne spores and given a dose of MPs a few hours before and/or after the spores. Pre-treatment with a single dose of CK-releasing MPs without any additional intervention was able to induce influx of neutrophils to the site of spore inoculation and regional lymph nodes correlating with reduced bacterial burden and decreased inflammatory response in footpads. On average, in two independent experiments, up to 53% of mice survived over 13 days. All control spore-challenged but MP-untreated mice died. The CB-coupled particles were also found to improve survival likely due to the capacity to stimulate release of endogenous CKs, but were less potent at decreasing the inflammatory host response than the CK-releasing MPs. The CK post-treatment did not improve survival compared to the untreated mice which died within 4 to 6 days with a strong inflammation of footpads, indicating quick dissemination of spores though the lymphatics after challenge. This is the first report on the enhanced innate host resistance to anthrax in response to CKs delivered and/or endogenously induced by the MPs. en_US
dc.description.sponsorship 1R21AI117425-01 from the National Institutes of Health, USA (SP VE and LL) en_US
dc.language.iso en_US en_US
dc.publisher PLOS en_US
dc.rights Attribution 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/us/ *
dc.subject Neutrophils en_US
dc.subject Bacterial spores en_US
dc.subject Inflammation en_US
dc.subject Mouse models en_US
dc.subject Anthrax en_US
dc.subject Immune Response en_US
dc.subject Inflammatory diseases en_US
dc.subject Immune cells en_US
dc.title Chemokine-Releasing Microparticles Improve Bacterial Clearance and Survival of Anthrax Spore-Challenged Mice en_US
dc.type Article en_US
dc.identifier.doi 10.1371/journal.pone.0163163


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