Mason Archival Repository Service

Global Gene Expression Analysis in an in vitro Fibroblast Model of Idiopathic Pulmonary Fibrosis Reveals Potential Role for CXCL14/CXCR4

Show simple item record

dc.contributor.author Rodriguez, Luis R.
dc.contributor.author Emblom-Callahan, Margaret
dc.contributor.author Chhina, Mantej
dc.contributor.author Bui, Sarah
dc.contributor.author Aljeburry, Bilal
dc.date.accessioned 2019-02-19T18:43:48Z
dc.date.available 2019-02-19T18:43:48Z
dc.date.issued 2018
dc.identifier.citation Rodriguez, Luis R., Margaret Emblom-Callahan, Mantej Chhina, Sarah Bui, Bilal Aljeburry, Luc H. Tran, Rebecca Novak, Merte Lemma, Steven D. Nathan, and Geraldine M. Grant. "Global Gene Expression Analysis in an in vitro Fibroblast Model of Idiopathic Pulmonary Fibrosis Reveals Potential Role for CXCL14/CXCR4." Scientific Reports 8, no. 1 (2018): 3983. en_US
dc.identifier.issn 2045-2322
dc.identifier.uri https://hdl.handle.net/1920/11397
dc.description.abstract Idiopathic Pulmonary Fibrosis (IPF) is a progressive disorder that is marked by an over accumulation of activated fibroblast populations. Despite the improved understanding of many mechanisms within this disease, global gene expression analysis has few focused studies on the fibroblast, the central effector cell of progressive fibrosis. We present a unique analysis of IPF pulmonary fibroblasts as they transition through cell culture and identify in vitro altered cellular processes. Fibroblasts were isolated from diseased (n = 8) and non-diseased (n = 4) lungs. Global gene expression analysis was carried out at the initial point of isolation and after 3 weeks of culture. We identify several genes that are altered by removal of the fibroblast from the IPF environment. Comparison of this subset of genes to four previously published whole lung analyses refined our list to a small subset of key fibroblast specific genes important in IPF. Application of STRING database analysis and confirmation via in-vitro and histological assay highlights the CXCL14/CXCR4 chemokine axis with a possible role in the progression and/or activation of fibroblasts within the IPF lung. Our findings, present a possible therapeutic target for IPF and a model for the study and discovery of novel protein and processes in this terrible disease.
dc.language.iso en_US en_US
dc.publisher Scientific Reports en_US
dc.rights Attribution 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/us/ *
dc.title Global Gene Expression Analysis in an in vitro Fibroblast Model of Idiopathic Pulmonary Fibrosis Reveals Potential Role for CXCL14/CXCR4 en_US
dc.type Article en_US
dc.identifier.doi 10.1038/s41598-018-21889-7


Files in this item

The following license files are associated with this item:

This item appears in the following Collection(s)

Show simple item record

Attribution 3.0 United States Except where otherwise noted, this item's license is described as Attribution 3.0 United States

Search MARS


Browse

My Account

Statistics