Mason Archival Repository Service

Computational Modeling for the Dysregulation of Calcium in Alzheimer Disease

Show simple item record

dc.contributor.advisor Ullah, Aman Rajora, Simaran
dc.creator Rajora, Simaran 2020-05-29 2021-09-22T00:06:23Z 2021-09-22T00:06:23Z
dc.description.abstract Alzheimer's disease (AD) is a devastating neurodegenerative disorder. According to Alzheimer Disease International (ADI), people develop dementia every 3 seconds in the world. ßA (Beta- Amyloid) is the main constituent of neuritic plaques. Amyloid beta (Aβ) accumulations at the intracellular level can increase the release of Ca2+ from the internal store endoplasmic reticulum (ER). Calcium is a very important signaling molecule where Ca2+ ion plays an important role in neuronal signaling. Abnormalities in calcium regulation have been reported in several neurodegenerative diseases. Increased oscillations of Ca2+ ion concentration is due to the most important cellular component known as IP3R. In this study 3-state model was used to describe all observed gating behavious in IP3R channels in Sf9 cells. Here, a simulated algorithm was adopted to optimize the parameters in a modified model by fitting the experimental results of open probabilities (Po) of IP3RPS1M146L saturates at significantly lower IP3 concentrations and its peak value is higher as compared to the IP3RPS1WT. By modifying a minimum number of model parameters, the 3-state model was applied to fit observed gating behaviors in channel Po. This research applied, IP3R stochastic model which was used to investigate the Ca2+ level puffs (amplitudes and duration) in normal cells and the one with AD cells. Investigating the model solution can provide important information on the impact of Aβ on Ca2+ basal levels at various timescales. Although it is unclear how Aβ disrupts the intracellular Ca2+ homeostasis there is an evidence that Aβ directly affects the production of IP3, through Ryanodine receptor (RyR) and plasma membrane by calcium-induced calcium release (CICR). We will use the results of these findings and validate them by computational modeling. Our future planning is to build a whole-cell model and check its effects on the elementary Ca2+ release events such as Puffs at the local and global levels. en_US
dc.language.iso en en_US
dc.subject IP3R en_US
dc.subject Wild Type en_US
dc.subject AD Type en_US
dc.subject Open Probability en_US
dc.subject Alzheimer en_US
dc.title Computational Modeling for the Dysregulation of Calcium in Alzheimer Disease en_US
dc.type Thesis en_US Master of Science in Bioinformatics and Computational Biology en_US Master's en_US Bioinformatics and Computational Biology en_US George Mason University en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search MARS


My Account