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SpliceCenter: a suite of web-based bioinformatics applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies

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dc.contributor.author Ryan, Michael C.
dc.creator Ryan, Michael C.
dc.date 2009-03-30
dc.date.accessioned 2009-05-15T17:18:17Z
dc.date.available NO_RESTRICTION en
dc.date.available 2009-05-15T17:18:17Z
dc.date.issued 2009-05-15T17:18:17Z
dc.identifier.uri https://hdl.handle.net/1920/4506
dc.description.abstract Alternative splicing of gene transcripts presents biologists with challenges that have largely been ignored to-date. Assays commonly used by researchers to explore biological processes including qRT-PCR, RNAi, expression microarrays, and peptide-based technologies target sequence fragments to quantify or silence gene expression. Alternate splicing may cause failures for these assays and complicates interpretation of resulting data particularly when correlation between platforms is attempted. For example, qRT-PCR may fail to validate expression microarray results if the PCR primers and microarray probes target different splice variants. The purpose of this project is to implement a suite of tools that assist biologists in quickly and easily identifying the impact of alternative splicing on commonly used laboratory techniques. It is hoped that these tools will lead to more effective use of these technologies and to more insightful interpretation of resulting data.
dc.language.iso en_US en
dc.subject Alternative Splicing en_US
dc.subject RNAi en_US
dc.subject Microarray en_US
dc.subject Transcript Isoforms en_US
dc.subject RT – PCR en_US
dc.subject Peptide Antibody en_US
dc.title SpliceCenter: a suite of web-based bioinformatics applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies en
dc.type Dissertation en
thesis.degree.name Doctor of Philosophy in Bioinformatics en
thesis.degree.level Doctoral en
thesis.degree.discipline Bioinformatics en
thesis.degree.grantor George Mason University en


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