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A search for KCNRG mutations in multiple myeloma cell lines

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dc.contributor.advisor Baranova, Ancha Coon, Stephanie
dc.creator Coon, Stephanie 2010-11-17 2011-02-21T21:34:37Z NO_RESTRICTION en_US 2011-02-21T21:34:37Z 2011-02-21
dc.description.abstract Deletions and/or rearrangements on chromosome 13q14.3 are observed in more than half of multiple myeloma (MM) and chronic lymphocytic leukemia (CLL) cases and are also frequently seen in other hematopoietic malignancies. The minimal common deleted region (CDR) in MM cells contains candidate tumor suppressor gene KCNRG (potassium channel regulating gene), the product of which suppresses assembly of the Kv channels and Kv currents. KCNRG exerts growth suppressive and pro-apoptotic effects in HL-60, LNCaP and RPMI-8226 cells. In this study, the KCNRG gene was sequenced in three multiple myeloma cell lines, NCI-H929, RPMI-8226 and U266B2. The RPMI-8226 cell line was found to contain a delT mutation in the core promoter initiator element. Deletion of T decreases matrix similarity of this DNA element from 0.945 to 0.941, and, therefore, might negatively influence expression of KCNRG in RPMI-8226 cells. The haploinsufficiency of KCNRG might be relevant to the progression of CLL and MM at least in a subset of patients.
dc.language.iso en_US en_US
dc.subject KCNRG en_US
dc.subject cancer en_US
dc.subject tumor suppressor en_US
dc.subject multiple myeloma en_US
dc.subject B-cell chronic lymphocytic leukemia en_US
dc.title A search for KCNRG mutations in multiple myeloma cell lines en_US
dc.type Thesis en Master of Science en_US Master's en Biology en George Mason University en

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