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Whole Proteome Analysis of Mouse Lymph Nodes in Cutaneous Anthrax

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dc.contributor.author Popova, Taissia G.
dc.contributor.author Espina, Virginia
dc.contributor.author Zhou, Weidong
dc.contributor.author Mueller, Claudius
dc.contributor.author Liotta, Lance
dc.contributor.author Popov, Serguei G.
dc.date.accessioned 2015-09-23T15:36:11Z
dc.date.available 2015-09-23T15:36:11Z
dc.date.issued 2014-10-20
dc.identifier.citation Popova TG, Espina V, Zhou W, Mueller C, Liotta L, Popov SG (2014) Whole Proteome Analysis of Mouse Lymph Nodes in Cutaneous Anthrax. PLoS ONE 9(10): e110873. doi:10.1371/journal.pone.0110873 en_US
dc.identifier.uri https://hdl.handle.net/1920/9902
dc.description.abstract This study aimed to characterize a soluble proteome of popliteal lymph nodes during lymphadenitis induced by intradermal injection of Bacillus anthracis Sterne spores in mice using tandem LC-MS/MS and reverse-phase protein microarray with antibodies specific to epitopes of phosphorylated proteins. More than 380 proteins were detected in the normal intra-nodal lymph, while the infectious process resulted in the profound changes in the protein abundances and appearance of 297 unique proteins. These proteins belong to an array of processes reflecting response to wounding, inflammation and perturbations of hemostasis, innate immune response, coagulation and fibrinolysis, regulation of body fluid levels and vascular disturbance among others. Comparison of lymph and serum revealed 83 common proteins. Also, using 71 antibodies specific to total and phosphorylated forms of proteins we carried initial characterization of circulating lymph phosphoproteome which brought additional information regarding signaling pathways operating in the lymphatics. The results demonstrate that the proteome of intra-nodal lymph serves as a sensitive sentinel of the processes occurring within the lymph nodes during infection. The acute innate response of the lymph nodes to anthrax is accompanied by cellular damage and inflammation with a large number of up- and down-regulated proteins many of which are distinct from those detected in serum. MS data are available via ProteomeXchange with identifier PXD001342.
dc.description.sponsorship This work was supported by the grant 5R21AI099851-2 from the National Institutes of Health, USA (SP, VE, and LL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publication of this article was funded in part by the George Mason University Libraries Open Access Publishing Fund. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.rights Attribution 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/us/ *
dc.subject lymph
 en_US
dc.subject serum proteins
 en_US
dc.subject anthrax
 
 en_US
dc.subject proteomes
 en_US
dc.subject STAT proteins
 en_US
dc.subject inflammation
 en_US
dc.subject phosphoproteins
 en_US
dc.subject bacterial spores en_US
dc.title Whole Proteome Analysis of Mouse Lymph Nodes in Cutaneous Anthrax en_US
dc.type Article en_US
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0110873


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