Presence of Noncoding RNA and Exosomal Biogenesis in HIV Infection
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Schwab, Angela
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Abstract
HIV-1 infection can be treated with antiretroviral drugs, but an efficient system in quantifying latent HIV infection is necessary in a clinical setting. This study shows how nanoparticles can be used to capture exosomes that contain HIV-1 RNA transcripts in patient serum, CVL, and CSF. Multiple viral RNA transcripts of different lengths were found in exosomes from infected cells, including a novel transcript termed “TAR-gag”. Exosomes from HIV-infected cells cause recipient cells to become more susceptible to future infection. This study also shows how siRNA can be used to knock down key proteins involved in the formation of exosomes to decrease the amount of viral RNA transcripts released in these exosomes.
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Keywords
HIV, Exosome, SiRNA, Virus, Nanoparticle, ESCRT