Abstract:
In forensic investigations, when an unknown decedent is found, the
postmortem interval is a critical data point in establishing identification
as well as reconstructing circumstances surrounding the death event. When the
body reaches the fourth stage of decomposition, advanced decay, the soft
tissues have been completely broken down. The decomposition of soft tissues
leads to skeletonization, where only the hard skeletal tissues remain. These
materials are then subject to diagenetic processes, including discoloration.
The most commonly encountered stains on bones are from soil, organic
materials, or metals. Staining on bone from metal compounds can be caused by
numerous circumstances wherein various types of metal from clothing,
projectiles, or other personal artifacts comes in contact with the remains.
Because several factors influence the rate of decomposition, the postmortem
interval between death and skeletonization can vary widely. Furthermore, the
methods for determining the time between skeletonization and discovery are
limited.
The following study explored the potential for estimating the
postmortem interval via copper and aluminum staining patterns on skeletal
remains. The specific goal of this research was to determine whether the
discolorations can assist in estimating time since skeletonization and
reconstructing the depositional environment. Additionally, the two types of
metals were compared to establish any distinct staining pattern or
discoloration on bone that is unique and can be presumed as belonging to
either copper or aluminum. In the experiment, seven deer tibias were buried
in a temperature-controlled environment. Pieces of copper and aluminum were
affixed to each tibia. Once a week, for 20 weeks, each bone was examined for
signs of discoloration from the metals. Munsell soil color charts were used
to quantify the observed skeletal color changes, and a qualitative scoring
system was used to measure the degree of staining each week. The staining on
bone caused by copper was predominantly green with some yellow and grey
variation and became more pronounced over time. Aluminum staining was largely
white and exhibited a lesser extent of color change. The data analysis
suggested both types of staining possess a rate of color change whose
variability is correlated with time. The results of this study will
contribute to the identification and assessment of discolorations on skeletal
remains, which can potentially help reconstruct the depositional scene and
estimate the time since skeletonization.