The Roles of LIM Kinase (LIMK) Inhibitors in the Regulation of Actin Dynamics During HIV-1 Infection

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dc.contributor.advisorWu, YuntaoGuo, Jia
dc.contributor.authorYi, Fei
dc.creatorYi, Fei
dc.date.accessioned2016-09-28T10:23:03Z
dc.date.available2016-09-28T10:23:03Z
dc.date.issued2016
dc.description.abstractA dynamic actin cytoskeleton is necessary for viral entry, intracellular migration, and virion release. For HIV-1 infection, during viral entry, the virus triggers early actin activity through hijacking chemokine coreceptor signaling which activates a viral dependency host factor cofilin and its kinase, the LIM domain kinase (LIMK). Although knockdown of human LIMK1 with siRNA inhibits HIV infection, no specific small molecule inhibitor of LIMK is available. Here we describe the design and discovery of novel classes of small molecule inhibitors of LIMK for inhibiting HIV infection. We identified R10015 as a lead compound that blocks LIMK kinase activity by binding to the ATP-binding pocket. R10015 specifically blocks viral DNA synthesis, nuclear migration, and virion release. In addition, R10015 inhibits multiple viruses including EBOV, RVFV, VEEV, and HSV-1, suggesting that LIMK inhibitors could be developed as a new class of broad-spectrum anti-viral drugs.
dc.format.extent135 pages
dc.identifier.urihttps://hdl.handle.net/1920/10450
dc.language.isoen
dc.rightsCopyright 2016 Fei Yi
dc.subjectVirology
dc.subjectMolecular biology
dc.subjectMicrobiology
dc.subjectActin
dc.subjectAntiviral drug
dc.subjectCofilin
dc.subjectHIV
dc.subjectKinase inhibitor
dc.subjectLIMK
dc.titleThe Roles of LIM Kinase (LIMK) Inhibitors in the Regulation of Actin Dynamics During HIV-1 Infection
dc.typeDissertation
thesis.degree.disciplineBiosciences
thesis.degree.grantorGeorge Mason University
thesis.degree.levelPh.D.

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