A Phylogenomic Analysis Of The Trumpeter (Cygnus buccinator) And Tundra Swans (Cygnus columbianus columbianus)
| dc.contributor.advisor | Gillevet, Patrick M. | |
| dc.contributor.author | Yesmont, Christopher Kenneth | |
| dc.creator | Yesmont, Christopher Kenneth | |
| dc.date | 2014-07-28 | |
| dc.date.accessioned | 2014-10-21T20:35:53Z | |
| dc.date.available | 2014-10-21T20:35:53Z | |
| dc.date.issued | 2014-10-21 | |
| dc.description.abstract | High-throughput or Next-Generation sequencing (NGS) has proven to be a time-efficient and cost-effective technique for the sequencing of whole-genomes. Despite its benefits, high-throughput sequencing is remaining a relatively nascent technique for phylogenetic analyses (McCormack 2013). We performed sequence assembly and analysis of genomic reads from a wild Tundra Swan (C. columbianus) and a wild Trumpeter Swan (Cygnus buccinator). The reads were assembled into Contigs that were later run through a custom Perl script designed to extract the differences between sequences. We also performed further analysis of the genetic relatedness between swan species using sequences of nuclear gene encoding the chromo-helicase-DNA binding domain (CHD) and associated introns that involved alignments and phylogenetic tree construction. Additionally, polymorphisms in the mitochondrial control region (D-loop) of Trumpeters, Tundra Swans, and hybrids were used for phylogenetic trees analysis. The phylogenetic analysis of the nuclear gene (CHD), D-loop, and genomic sequences indicates strong introgression between the two species due to extensive hybridization. | |
| dc.identifier.uri | https://hdl.handle.net/1920/9094 | |
| dc.language.iso | en_US | |
| dc.rights | Copyright 2014 Christopher Kenneth Yesmont | |
| dc.subject | Trumpeter swan | |
| dc.subject | Tundra swan | |
| dc.subject | Introgression | |
| dc.subject | Hybridization | |
| dc.subject | Phylogenomics | |
| dc.subject | Next-generation sequencing | |
| dc.title | A Phylogenomic Analysis Of The Trumpeter (Cygnus buccinator) And Tundra Swans (Cygnus columbianus columbianus) | |
| dc.type | Thesis | |
| thesis.degree.discipline | Biology | |
| thesis.degree.grantor | George Mason University | |
| thesis.degree.level | Master's | |
| thesis.degree.name | Master of Science in Biology |