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Oxidized Extracellular DNA as a Stress Signal in Human Cells

Show simple item record Ermakov, Aleksei V. Konkova, Marina S. Kostyuk, Svetlana V. Izevskaya, Vera L. Baranova, Ancha Veiko, Natalya N. 2014-09-12T23:46:28Z 2014-09-12T23:46:28Z 2013-01
dc.identifier.citation Aleksei V. Ermakov, Marina S. Konkova, Svetlana V. Kostyuk, Vera L. Izevskaya, Ancha Baranova, and Natalya N. Veiko, “Oxidized Extracellular DNA as a Stress Signal in Human Cells,” Oxidative Medicine and Cellular Longevity, vol. 2013, Article ID 649747, 12 pages, 2013. doi:10.1155/2013/649747 en_US
dc.identifier.other doi:10.1155/2013/649747
dc.description.abstract The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increase. Dying cells release their damaged DNA, thus, contributing oxidized DNA fragments to the pool of cfDNA/ecDNA. Oxidized cell-free DNA could serve as a stress signal that promotes irradiation-induced bystander effect. Evidence points to TLR9 as a possible candidate for oxidized DNA sensor. An exposure to oxidized ecDNA stimulates a synthesis of reactive oxygen species (ROS) that evokes an adaptive response that includes transposition of the homologous loci within the nucleus, polymerization and the formation of the stress fibers of the actin, as well as activation of the ribosomal gene expression, and nuclear translocation of NF-E2 related factor-2 (NRF2) that, in turn, mediates induction of phase II detoxifying and antioxidant enzymes. In conclusion, the oxidized DNA is a stress signal released in response to oxidative stress in the cultured cells and, possibly, in the human body; in particular, it might contribute to systemic abscopal effects of localized irradiation treatments.
dc.description.sponsorship This work was supported by the RFBR (12-04-32081), by the Contract no. 8273 (August 27, 2012) under the call no. 2012-1.1-12-000-2008-067 of the Ministry of Education and Science of Russia, and by Thomas F. Jeffress and Kate Miller Jeffress Foundation Grant J-1023. en_US
dc.language.iso en_US en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.rights Attribution 3.0 United States *
dc.rights.uri *
dc.subject Adaptive responses en_US
dc.subject Bystander effect en_US
dc.subject DNA double-strand breaks en_US
dc.subject Extracellular DNA en_US
dc.subject Oxidative stress en_US
dc.subject Reactive oxygen species en_US
dc.subject Human umbilical vein endothelial cells en_US
dc.subject Mesenchymal stem cells. en_US
dc.title Oxidized Extracellular DNA as a Stress Signal in Human Cells en_US
dc.type Article en_US

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